Optical Micro-Spectroscopy (OptiMis) for spectrally resolved fluorescence imaging
OptiMiS may be used in conjunction with FRET for quaternary protein structure determinations.
Multiple fluorophores and multiple excitation wavelengths
If more than two fluorescent tags are employed, it is useful for a microscope system to present spectral resolution not only in the detection but also in the excitation channel.
Rapid Scanning Systems
Monitoring dynamic protein-protein interactions at single molecule level requires rapid acquisition speed.